Cell Line Development Cell Culture Purification Formulation Analytical Development
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Cell Line Development
Cell Line Development

To obtain the best results for a wide range of cell culture products, Bioworkshops is agnostic when it comes to expression system. We cooperate with many leading cell line development suppliers as well as offering multiple CHO-based expression platforms of our own. Our cell culture leaders will evaluate key indicators of the product to recommend the best cell line construction plan and screening process. Starting from target molecule, DNA or antibody sequence we provide all the services from the vector construction to the screening of stable and high-expressing cell lines that meet the required protein quality for reliable commercial production.

Cell Line project deliverables

Vector construction

Cell transfection

Cell pool/ Minipool screening

Single clone screening

RCB cell banking

RCB stability study

Fully documented history for registration dossier.

Cell Line development process

  • ~3 weeks

    Vector construction

    Select suitable vector

    Codon optimization

    Gene synthesis

    Plasmid construction

    Target sequence sequencing

  • ~6 weeks

    Cell transfection and cell pool/ Minipool screening

    Select suitable transfection conditions

    Monitor growth and expression 48h after transfection

    Cell pool/ Minipool preparation

    Top pool/ fed-batch (FB) cell performance assay

    Top pool/ Minipool quality analysis

  • 7~8 weeks

    Single clone screening

    Top pool/ Minipool single clone preparation

    Clone images

    Top single clone Fed batch cell performance assay

    Single clone quality analysis

  • ~13-14 weeks

    RCB banking and stability study

    Top 3 RCB Cell Banking (30 vials)

    RCB mycoplasma test

    60 PDL stability study

    FB evaluation every 15~20 PDL

    Quality inspection of samples at the start and end points

    Quality inspection of samples at the start and end points (Copy number and target gene sequencing)

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