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  • Vector construction

    Posted 23 Sep 2021|Part time|Cell Line development process

    Select suitable vector

    Codon optimization

    Gene synthesis

    Plasmid construction

    Target sequence sequencing

  • Cell transfection and cell pool/ Minipool screening

    Posted 23 Sep 2021|Part time|Cell Line development process

    Select suitable transfection conditions

    Monitor growth and expression 48h after transfection

    Cell pool/ Minipool preparation

    Top pool/ fed-batch (FB) cell performance assay

    Top pool/ Minipool quality analysis

  • Single clone screening

    Posted 23 Sep 2021|Part time|Cell Line development process

    Top pool/ Minipool single clone preparation

    Clone images

    Top single clone Fed batch cell performance assay

    Single clone quality analysis

  • RCB banking and stability study

    Posted 23 Sep 2021|Part time|Cell Line development process

    Top 3 RCB Cell Banking (30 vials)

    RCB mycoplasma test

    60 PDL stability study

    FB evaluation every 15~20 PDL

    Quality inspection of samples at the start and end points

    Quality inspection of samples at the start and end points (Copy number and target gene sequencing)

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